摘要
采用毛细管区带电泳法测定了 10种中国产葡萄籽中的 4个主要儿茶素类化合物 :(+)儿茶素、(- )表儿茶素、(± )表没食子儿茶素、(± )表儿茶素没食子酸酯的含量。在 0 0 2mol/L硼砂和 0 0 0 5mol/L磷酸盐的混合缓冲体系 (pH 10 0 )的背景缓冲液中 ,4个化合物在 10min内取得了令人满意的分离。迁移时间的重现性(RSD)小于 2 % ,峰面积的重现性 (RSD)小于 5 %。在质量浓度为 0 0 0 5g/L～ 0 5 g/L时 ,线性相关系数大于0 995。检测限为 3mg/L～ 10mg/L。该方法简单、快速、准确 。
A capillary zone electrophoresis(CZE) method has been developed for the determination of four pharmacologically active catechins in grape seed The components analyzed were (+) catechin, (-) epicatechin, epigallocatechin and epicatechin gallate Separation was achieved by using a mixed buffer of 0 02 mol/L borate and 0 005 mol/L phosphate at pH 10 0 as background electrolyte and an uncoated fused silica capillary with 50 μm i d ×375 μm o d (effective length 41 5 cm ) maintained at 25 ℃ The applied voltage was 25 kV, and detection was performed by DAD at wavelength of 210 nm By using proposed CZE method, four catechins were well separated within only 10 minutes with the repeatability of <1% RSD for migration time and <5% RSD for peak area Correlation coefficients better than 0 995 were obtained within the concentration range from 0 005 g/L to 0 5 g/L for the four analytes studied with the detection limit of 3 mg/L 10 mg/L The contents of these compounds in ten species of grape seeds cultivated in China were successfully determined.
出处
《色谱》
CAS
CSCD
北大核心
2000年第6期491-494,共4页
Chinese Journal of Chromatography
基金
国家自然科学基金资助项目! (批准号 :2 9875 0 0 1)
关键词
毛细管区带电泳
葡萄籽
儿茶素
表儿茶素
测定
capillary zone electrophoresis
grape seed
(+) catechin
(-) epicatechin
epigallocatechin
epicatechin gallate
