摘要
目的 :建立测定七味解毒活血膏中儿茶素含量的HPLC方法 ,用以控制七味解毒活血膏的质量。方法 :色谱柱为C1 8柱 ;流动相为乙腈 - 0 . 2 %磷酸 (8∶92 ) ;检测波长为 2 80nm ;流速 1 0ml/min。结果 :儿茶素在0 .0 0 2 75～ 1 . 375 μg范围内线性关系良好 (r =0 . 99999) ,平均加样回收率为 98. 94 %,(RSD =1 . 1 0 %,n =6 )。结论 :本法灵敏、方法简便、准确 ,可用于七味解毒活血膏的质量控制。
Objective:To establish a HPLC method for the determin ation of Cat echin in Qiweijieduhuoxie Gao.Methods:The chromatographic conditions were C 1 8 column;mobile phrase of CH 3CN-0.2%H 3PO 4(8∶92);flow rate of 1.0 ml /min and detection wavelength at 280nm.Results:The standard curve of Catechin wa s linear over the range of 0.00275～1.375μg with the correlation coefficient 0.99999.The average recovery was 98.94%with RSD=1.10%(n=6).Conclusion:The HP LC method is sensitive,rapid and accurate.It can be used to control the quality of Qiweijieduhuoxie Gao.
出处
《云南中医学院学报》
2005年第1期16-17,30,共3页
Journal of Yunnan University of Traditional Chinese Medicine
