摘要
目的:研究刚果红与卡那霉素的褪色反应,建立一个简单、灵敏、选择性高的微量硫酸卡那霉素的测定方法。方法:分光光度法。结果:在pH5.02的Britton-Robinson(B-R)缓冲溶液中,刚果红与硫酸卡那霉素相互作用而导致溶液吸收光谱的变化,用分光光度法对光谱变化进行了研究。刚果红溶液在500 nm处有一强的特征吸收峰,当在其溶液中加入硫酸卡那霉素后,溶液发生褪色反应,500 nm处吸收峰强度降低,吸光度差值与硫酸卡那霉素浓度成正比。最佳条件下,卡那霉素的浓度在0.1-3.0 mg/L范围内遵守比尔定律,表观摩尔吸光系数ε=6.8×104L.mol-1.cm-1,检测限(3σ)为0.079 mg/L,平均回收率为96.15%。将该方法应用于硫酸卡那霉素注射液的测定,结果满意。结论:刚果红光度法可用于微量硫酸卡那霉素的定量分析。
Objective： To observe the discoloration reaction between kanamycin sulfate and Congo red to find a simple and sensitive method with high selectivity for the determination of trace kanamycin. Methods： Spectrophotometry was adopted. The interaction of kanamycin sulfate and Congo red was observed in pH = 5.02 Britton Robinson buffer solution. Results： Congo red has a strong absorbance at 500 nm and the addition of kanamycin sulfate into Congo red solution resulted in the decrease of absorbance value, and the decrease amount was linear with the concentration of kanamycin sulfate. Under the optimal conditions, Beer＇ s law was obeyed in the range of 0.1 - 3.0 mg/L, with apparent molar absorptivity of 6.8 ×10^L · mol^-1 · cm^-1 and the detection limit of 0.079 mg/L;The recovery rate was 96.15%. Conclusions： We obtained satisfactory results in determination of sodium injection solution with this method, which shows this method could be applied in the determination of trace kanamycin sulfate.
出处
《贵阳医学院学报》
CAS
2007年第1期50-52,共3页
Journal of Guiyang Medical College
