摘要
目的测定异甘草素固体脂质纳米粒中低分子肝素钠(LMWH)的包封率。方法在Britton-Robinson(B-R)缓冲溶液中,天青A与LMWH相互作用,在662 nm处测定吸光度差值(△A),用△A对LMWH浓度进行线性回归。样品同法操作,测定LMWH的包封率。结果 LMWH的回收率在90.0%～102.5%之间,线性范围为100～1 000μg/mL(r=0.999 1),异甘草素固体脂质纳米粒中LMWH的平均包封率为35%(n=5)。结论此法操作简单、准确,适用于该制剂的质量评价。
Purpose To determine the encapsulation efficiency of low molecular weight heparin（LMWH）in isoliquiritigenin（ISL）nanoparticle.Methods In Britton-Robinson（B-R）buffer solution,Azure A chloride Salt has a maximum absorption peak at 662 nm and the absorbance reduced by the reaction with LMWH.Polynomial regression between absorbance and LMWH concentration was made.ISL nanoparticle and ultrafiltrate were treated by the similar method and absorbance was recorded.The contents of LMWH and entrapment efficiency were calculated respectively.Results Recoveries of LMWH were between 90.0%-102.5%,the linear ranges of determination of LMWH was 100-1 000 μg/mL（r=0.999 1）.Average encapsulation efficiency of LMWH in ISL nanoparticle was 35%（n=5）.Conclusion The method was convenient and accurate.It is suitable for the quantity control of ISL nanoparticle.
出处
《中国生化药物杂志》
CAS
CSCD
北大核心
2012年第4期381-384,共4页
Chinese Journal of Biochemical Pharmaceutics
基金
甘肃省中医药科研立项课题(GZK-2008-21)
关键词
异甘草素
固体脂质纳米粒
低分子量肝素钠
天青A
纳米粒
包封率
isoliquiritigenin
nanoparticle
low molecular weight heparin
Azure A chloride salt
nanoparticle
encapsulation efficiency
