摘要
用几种氨基酸铜溶液处理大丽轮枝菌,阴离子交换层析分离培养液中的毒素蛋白,并进行棉苗的致萎实验,比较了各氨基酸铜制剂对大丽轮枝菌毒素蛋白产生的影响。实验表明,丙氨酸铜、甘氨酸铜、谷氨酸铜和复合氨基酸铜都在一定程度上抑制了大丽轮枝菌微菌核的形成和毒素蛋白的分泌,而苏氨酸铜促进了毒素蛋白的分泌。说明复合氨基酸铜的抑菌作用是各成分协同作用的结果。
Verticillium wilt of cotton plant which infected by Verticillium dahliae is a kind of plant fungal disease in the vascular bundle. In this research, we used cupric complexes of alanine, cupric complexes of glyeine, cupric complexes of glutamie acid, cupric complexes of threonine and cupric complexes of mixed aminoacid to test the inhibition on the Verticillium dahlia. These five fungicides were developed by our laboratory as new kinds of antiseptic. Verticillium dahliae was cultured in the Czapeek＇s culture solution which added the cupric complexes of amino-acid at 25℃ and the control added aseptic water. After 20 days we extracted the secreted protein of Verticillium dahliae cultured in the solution, then isolated the protein by anion-exchange chromatography, and the area of the chromatographic peak can indicate the amount of every collection. At the same time, we collected every isolated parts, respectively, to test the activity. In the wilt-test, we cultured the cotton seedlings in a certain nutrition solution, after they grew out four pieces of leaves, we selected the healthy seedlings as the wilt-test materials, the phytotoxic protein was added in the nutrition solution culturing cotton seedlings, then we observed the growth of the seedlings in 24-72 h. By analyzing the chromatographic peak and the wilt-test accordingly, it showed that cupric complexes of alanine, cupric complexes of glycine, cupric complexes of glutamic acid and cupric complexes of mixed amino-acid could inhibit the formation of microsclerofia and reduce the secretion of the phytotoxic protein of Verticillium dahliae, but cupric complexes of threonine increased the secreted protein. Furthermore , neither of the wilt-tests showed that the five fungicides could inhibit the activity of the phytotoxic protein. All results indicated that the inhibition of the five cupric complexes of amino-acid antiseptic on the verticillium wilt of cotton plant by the decrease of the amount of the phytotoxicprotein. But the reason that cupric complexes of threonine increased the secreted protein is not clear. To compare the inhibiting effect of cupric complexes of mixed amino-acid and the four single ingredient fungicides, it showed that all kinds of cupric complexes of amino-acid had different effects on the Verticillium dahliae, so the ingredients in the cupric complexes of mixed amino-acid interacted cooperatively to inhibit Verticillium dahliae.
出处
《棉花学报》
CSCD
北大核心
2006年第2期79-82,共4页
Cotton Science
基金
河北省自然科学基金资助项目(398152)
关键词
大丽轮枝菌
黄萎病
毒素蛋白
致萎活性
微菌核
蛋白液相色谱
Verticillium dahliae
Verticillium wilt
phytotoxic protein
wilt activity
microsclerotium
protein liquid chromatography
