摘要
从黄萎病发病棉田未发病棉株根际土样中分离芽孢杆菌,利用对峙培养法对大丽轮枝菌进行平板拮抗实验,筛选出6株对大丽轮枝菌有拮抗作用的芽孢杆菌,其中3株菌发酵代谢产物具有抗菌活性。S4-3菌株发酵液滤液经100℃、30min热处理丧失抗菌活性,发酵液氯仿抽提物不具有抗菌活性,发酵液硫酸铵沉淀物有抑菌活性,但经蛋白酶处理后活性丧失,认为S4-3菌株抑菌活性物质是蛋白质。而菌株S4-5发酵液经100℃热处理后还有较高的抗菌活性,发酵液氯仿抽提物也具有抗菌活性,但硫酸铵沉淀物对病原菌无抑制作用;S5-6菌株发酵液滤液经100℃热处理丧失抗菌活性,硫酸铵沉淀物也不具有抗菌活性,而发酵液氯仿抽提物有抗菌活性,因此认为S4-5和S5-6菌株产生的抑菌物质不是蛋白质类。
Thirty spore-forming bacterium strains were isolated from several samples of soil in cotton field. Antagonistic tests of all these strains against Verticillium dahliae were carried out on PDA medium by pairing culture method. The results showed that there were six strains which had antagonistic activity against Verticilliurn dahliae and of the six strains, there were three strains whose metabolite had the antagonistic activity. After treated with （NH4）2SO4 fractional precipitation, the liquid fermentation production of S4-3 strain showed antagonistic activity against Verticillium dahliae. And the antagonistic activity was lost after treated with heat（100℃,30 min） and CHCl2. It is suggested that the antifungal substance of the S4-3 strain should be protein. The antagonistic activity of liquid fermentation production of the S4-5 strain remained relatively high after treated with heat （100℃ ,30 min）, and the chloroform extracts of liquid fermentation production of the S4 5 strain showed the antagonistic activity too. But after treated with （NH4）2SO4 fractional precipitation, the liquid fermentation production of strain S4-5 did not show the antagonistic activity to Verticillium dahliae. The antagonistic activity of liquid fermentation production of the S5-6 strain was lost after treated with heat （100℃ ,30 min）, but the chloroform extracts of liquid fermentation production of the S5 6 strain still had the antagonistic activity. In addition, after treated with （NH4）2SO4 fractional precipitation, the S5-6 strain did not show the antagonistic activity, either. All these results indicated that the antifun-gal substance of S4-5 strain and S5-6 strain must not be protein.
出处
《棉花学报》
CSCD
北大核心
2008年第6期442-446,共5页
Cotton Science
基金
河北省自然科学基金项目(398152)
关键词
棉花黄萎病
拮抗细菌
分离
抗菌物
鉴定
Cotton Verticillium wilt
antagonistic bacterium
screening
antifungal substance
identification
