摘要
为提高棉花黄萎病拮抗细菌HMB-1005的芽孢形成率及芽孢数量,分析摇瓶发酵对芽孢形成的主要影响因素.通过单因素试验和正交试验对HMB-1005菌株产芽孢条件进行分析,确定最佳摇瓶发酵条件为:蔗糖1%、玉米粉2%、CaCl2·2H2O0.02%和NaH2PO4·2H2O0.2%、pH值为7.0、温度为30℃、转速为200r/min、接种量10%、种龄24h。在此优化条件下,发酵液中拮抗菌出芽率达到90%以上.
In order to improve the sporulation rate and spore quantity of antagonistic strain HMB-1005 against Verticillium dahliae. The authors investigated the main factors influencing sporulafion using shake-flask fermentation method. Through single factor experiment and orthogonal experiment,the optimal shaking flask fermentation condition was determined as follow： media composed of 1% sucrose,2 % maizena,0.02% CaCl2 ·2H2O and 0.2 % NaH2PO4·2H2O, initial pH 7.0 and 10 % inoculum volume,fermentation temperature 30 ℃ ,rotating speed 200 r/min,incubation time 24 h. Under this optimum condition,the germination rate exceeds 90 percentages.
出处
《保定学院学报》
2008年第2期31-34,共4页
Journal of Baoding University
基金
河北省自然科学基金(398152)
关键词
棉花黄萎病
拮抗细菌
芽孢
发酵条件
Verticillium dahliae
antagonistic bacterium
spore
fermentation conditions
