摘要
为了对棉花黄萎病生物防治的进一步研究,筛选出一株编号12-47的对大丽轮枝菌具有拮抗作用的菌株并设计了该实验对其实验室发酵条件进行了摸索。笔者采用单因素实验的方法在摇床培养的基础上对影响棉花黄萎病拮抗细菌12-47菌株产生拮抗物质的营养因素进行了考察,并在此基础上通过正交试验对各因素的浓度和组合进行了优化;通过L16(43)正交实验对培养最佳培养基条件进行了摸索。由实验数据笔者确定优化后的培养基为:碳源玉米粉3%、氮源大豆蛋白胨5%、无机盐Mn2+0.1%、K+0.05%、起始pH8.0、装液量100ml/250ml三角瓶、接种量8%、发酵时间48h。最终通过优化后的培养基进行发酵,发酵液抑菌活性比优化前提高了269%。
The fermentation conditions of the antagonistic bacteria 12-47 to cotton wilt were studied by using the single-factor method.The main factors affecting fermentation conditions include C and N sources,inorganic salts,pH,temperature,rotation speed.The best fermentation condition of each factor were obtained as follows：C source fructose,N source casein protein,inorganic salts MnSO4 and K2HPO4,pH8.0,inoculation volume 8%,seed culture time 72 h.The concentration of fermentation conditions were optimized through orthogonal design and the final fermentation medium have more fermentation productions compared with some other common medium.The final optimization of the medium：fructose 3%,casein protein 5%,0.1% Mn2＋,K＋ 0.05%,pH 8.0,shaking flask volume is at 100 ml/250 ml,Inoculation volume 8%,the time of fermentation is 48 hours.Antibacterial activity raise 269% after fermentation than before.
出处
《中国农学通报》
CSCD
2008年第12期68-73,共6页
Chinese Agricultural Science Bulletin
基金
河北省自然科学基金项目"从棉花黄萎病拮抗细菌中分离纯化抗菌蛋白基因"(398152)
关键词
大丽轮枝菌
拮抗菌
发酵条件
优化
Verticillium dahliae kleb.,antagonistic bacteria,optimization,fermentation conditions
