摘要
在河南省不同地区分离并鉴定33株棉花黄萎病菌分离物,并进行了致病力和粗毒素致萎活性测定。致病力结果表明,河南省棉花黄萎病菌分离物可分为强、中、弱3种致病力类型,分别占48.5%、21.2%、30.3%。河南省同一地区内不同分离物间致病力差异明显。粗毒素致萎活性测定结果表明,黄萎病菌强致病力分离物分泌的粗毒素对棉苗的致萎力较强,弱致病力分离物分泌的粗毒素对棉苗的致萎力较弱,强、中、弱3种致病力类型分离物在72h时对棉苗的平均致萎指数分别为63.82、52.38、45.83。采用考马斯亮蓝法测定不同分离物粗蛋白含量,结果表明,不同黄萎病菌分离物分泌的粗蛋白含量存在明显差异,且与其所致的病情指数呈显著正相关。
In order to research pathogenicity differentiation of Verticillium dahliae on cotton in Henan, 33 Verticillium dahliae isolates were isolated and identified from the main cotton-producing regions in Henan Province. The results of pathogenicity tests on cotton seedings indicated that the V. dahliae isolates from cotton in Henan could be divided into three pathotypes： the highly aggressive isolates, the moderate aggressive ones and the weakly aggressive ones, accounting for 48.5%, 21.2% and 30.3%, respectively. The results also showed that the pathogenicity of isolates had no significant difference among different regions, but the pathogenicity of isolates from the same region showed significant difference. The wilting capability of the crude toxin secreted by V. dahliae isolates were also tested, the results showed that the crude toxin of different isolates had different wilting capability; the crude toxin secreted by the highly aggressive isolates had strong wilting capability. The average wilt index of the highly aggressive isolates, moderate aggressive ones and weakly aggressive ones at 72 h were 63.82, 52.38 and 45.83 respectively at the same concentration. The crude protein contents secreted by different isolates were also tested by the Coomassie Brilliant Blue G250 method. The crude protein contents of different isolates of V. dahliae had a significant positive correlation with the pathogenicity index. Highly aggressive isolates secreted more proteins than the weakly aggressive ones.
出处
《棉花学报》
CSCD
北大核心
2010年第4期333-338,共6页
Cotton Science
基金
国家自然科学基金项目(30700520)
农业部公益性行业科研专项(nyhyzx07-052)
河南自然科学基金(2008A208015)
河南高校博士启动基金项目(30400229)
关键词
棉花黄萎病
大丽轮枝菌
致病力
毒素
cotton Verticillium wilt
Verticillium dahliae
pathogenicity
toxin
