摘要
目的探讨急性髓系白血病(AML)患者中上皮钙黏附素(E-cadherin)基因表达及其甲基化状态。方法分别用RT-PCR和流式细胞术方法检测55例AML患者骨髓细胞和7份正常对照骨髓细胞中E-cadherin基因和蛋白的表达,并用甲基化特异性PCR方法分析E-cadherin基因5’端CpG 岛的甲基化状况。结果 55例AML患者骨髓细胞中E-cadherin mRNA和蛋白表达阳性率分别为 23．6％和18．2％,较正常对照组明显下调(P值均<0．01);E-cadherin基因5’端甲基化的阳性率为 69．1％。E-cadherin mRNA和蛋白表达阴性的31例患者中,29例(93．5％)E-cadherin甲基化阳性,其蛋白水平和mRNA表达水平与启动子甲基化呈明显负相关(P<0．01)。7份正常对照骨髓细胞E-cad- herin mRNA和蛋白表达均为阳性,E-cadherin基因的CpG岛无明显甲基化。结论 AML的粒系分化成熟障碍可能与E-cadherin基因表达下调有关,5’端CpG岛的甲基化可能影响E-cadherin基因表达。
Objective To study the relationship between E-cadherin gene expression and the methylation status of E-cadherin 5＇ CpG islands in acute myeloid leukemia（AML）. Methods Reverse transcription-PCR（ RT-PCR）, flow cytometry and methylation specific PCR were used to analyze the E-cadherin gene and protein expression and its 5＇ CpG island methylation status respectively in bone marrow cells from 55 AML patients and 7 normal controls, Results AML cells displayed a significant reduction or lack of E-cadherin gene and protein expression, the positive rates were 23.6% and 18.2% （P 〈0.01 ）, respectively, All normal bone marrow cells were E-cadherin positive. Thirty-eight of the 55 patients （69.1% ） were E-cadherin 5＇ CpG island methylated whereas the normal controls were completely unmethylated. Twenty-nine of thirty-one （93.5%） E-cadherin-negative samples showed abnormal hypermethylation of the E-cadherin CpG islands, Conclusion Expression downregulation and methylation of E-cadherin gene in AML suggest that it might be an important event in AML. E-cadherin methylation was associated with the inhibition of E-cadherin gene and protein expression in AML,
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2006年第1期25-27,共3页
Chinese Journal of Hematology
基金
辽宁省科技攻关计划资助项目(2004225004-101)
