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E-cadherin在白血病细胞的表达及机制 被引量：5

Expression of E-cadherin in leukemic cells and its mechanism
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摘要 目的:检测E-cadherin在白血病细胞的表达并探讨其机制,最终阐明E-cadherin介导的造血细胞与骨髓基质间的黏附改变在白血病发生中的作用。方法:应用半定量RT-PCR方法检测98例白血病患者和23例正常骨髓移植供者骨髓单个核细胞的E-cadherin相对表达水平,应用半定量RT-PCR和甲基化特异性PCR(MSP)方法检测各种类型白血病细胞系E-cadherin的表达。用去甲基化试剂5-Aza-CdR处理E-cadherin完全甲基化的白血病细胞系U937,MSP、RT-PCR、流式细胞术及免疫荧光标记法检测诱导后的E-cadherin表达水平。结果:和正常人相比,98例白血病患者E-cadherin表达降低或缺失,非配对t检验进行统计学分析,P<0.05,差异显著;所有白血病细胞系E-cadherin表达缺失或降低,并且其基因处于完全甲基化状态或以甲基化为主的不完全甲基化状态。在合适的5-Aza-CdR作用浓度诱导下,部分细胞在mRNA和蛋白水平恢复E-cadherin表达。结论:白血病细胞E-cadherin表达降低或缺失,其基因的甲基化是重要机制,去甲基化处理可以使部分细胞重新表达E-cadherin。
作者 孟继虹 饶青 王敏 王东海 李敏 王洋 田征 唐克晶 王建祥
机构地区 中国医学科学院血液学研究所
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2008年第10期2044-2047,共4页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No.30671096)
关键词 钙粘着蛋白类 白血病 甲基化 5-Aza—CdR Cadherins Leukemia Methylation 5-Aza CdR
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献11

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二级参考文献12

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共引文献13

同被引文献43

  • 1高峰,李艳,刘巍,卢香兰,李霞,王萍萍,刘云鹏.急性髓系白血病E-cadherin基因表达和CpG岛甲基化状态的研究[J].中华血液学杂志,2006,27(1):25-27. 被引量：9
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引证文献5

二级引证文献18

中国病理生理杂志
2008年 第10期

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