摘要
目的:建立蝎子七药材的质量控制方法。方法:采用薄层色谱法定性;高效液相色谱法定量,色谱条件为 Hypersil C_(18)柱(250 nm×4.6mm,5μm),甲醇-水-磷酸(5:95:0.5)为流动相,检测波长271 nm,流速1.0 mL·min^(-1),柱温30℃。结果:没食子酸峰与其他峰分离良好。线性范围为20.02～600.6 ng,与峰面积呈良好的线性关系。加样回收率为98.17%,RSD 为1.1%(n=5)。结论:本方法简便快速,灵敏度、精密度和稳定性良好,能有效控制蝎子七药材的质量。
Objective： To establish the effective methods for the quality control of Rhizoma Polygom vivipari. Methods： Gallic acid was identified by TLC. The contents of gallic acid were determined by HPLC, carried on a column of Hypersil C 18 （250 mm × 4. 6 mm,5 μm） with a mobile phase of methanol - water - phosphoric acid （5： 95： 0. 5 ）, the detection wavelength was at 271 nm,with flow rat at 1. 0 mL· min^ -1 ,the temperature of the column was 30 ℃. Results： The TLC methods were simple wish strong specificity and good reproducibility. The contents of gallic acid were dertermined by HPLC, with accuracy of the average recoveries of 98. 17% （RSD = 1%, n = 5 ）. Conclusion： These methods can be used for quality control of Rhizaoma Polygom vivipari effectively.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第11期1343-1345,共3页
Chinese Journal of Pharmaceutical Analysis
