摘要
目的观察异丙酚对大鼠局灶性脑缺血和脑内蛋白激酶Cγ亚单位(PKCγ)变化的作用。方法♂SD大鼠随机分为Ⅰ:假手术组,Ⅱ:损伤组,Ⅲ:异丙酚(25mg·kg-1)+损伤组,Ⅳ:异丙酚(50mg·kg-1)+损伤组,Ⅴ:脂肪乳剂+损伤组,每组8例。采用大脑中动脉线栓法缺血3h再灌注24h。异丙酚和脂肪乳剂于再灌注前30min腹腔注射。通过原位末端脱氧核苷酸转移酶标记法观察局灶性脑缺血产生的神经细胞凋亡和异丙酚作用效果,免疫细胞化学法观察各组PKCγ蛋白在脑内表达变化的差异。结果再灌注24h后Ⅱ、Ⅲ、Ⅳ、Ⅴ组大鼠体重较缺血前降低(P<0.01),和Ⅰ组比较:Ⅱ、Ⅲ、Ⅳ、Ⅴ组大鼠再灌注24h后出现明显的神经体征缺陷(P<0.01),Ⅱ组大鼠额叶皮层和纹状体区域大量神经细胞凋亡,纹状体PKCγ蛋白表达明显下降。Ⅱ、Ⅲ、Ⅳ、Ⅴ组大鼠纹状体区域凋亡细胞密度与PKCγ染色阳性面积均没有差异(P>0.05)。结论再灌注前30min腹腔注射异丙酚对大鼠局灶性脑缺血再灌注损伤所致的神经细胞凋亡和PKCγ表达降低无保护作用。
Aim To investigate the effects of propofol on focal cerebral ischemia and the changes of protein kinase C isoform γ （PKCγ） expression in rats brain. Methods Male SD rats were randomly allocated into five groups. Ⅰ ： sham group, Ⅱ： injury group, Ⅲ： propofol （25 mg ·kg^-1） plus injury group, Ⅳ： propofol （50 mg· kg^-1） plus injury group, Ⅴ： intralipid plus injury group. The focal cerebral ischemia was induced by 3 h of middle cerebral artery occlusion （MCAO） and 24 h of reperfusion. 30 min before reperfusion, propofol and intralipid were infused intra- peritoneal of the rats in groups Ⅲ, Ⅳ, Ⅴ, respectively. After 24 h of reperfusion, rats were weighted and the neurological deficit was assessed by 5-point scale. Brain pathologic changes were observed by HE staining, the method of terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling （TUNEL） was carried out for the assessment of neural apoptosis, and immunocytochemistry was used to investigate the changes of PKCγ Results After 3 h of MCAO and 24 h of reperfusion, the weight of rats in group Ⅱ , Ⅲ, Ⅳ, Ⅴ decreased and their neurological deficits scores increased. Compared to the rats of sham group, the numbers of apoptosis neurons in striatum were markedly increased, and the expression of PKCγ were significantly decreased in rats of injury group （ P 〈 0. O1 ） Propofol and intralipid had no effects on the increasing of apoptosis neurons and the decreasing of PKCγ expression induced by focal cerebral ischemia. Conclusions Propofol has no neuroprotective effects on focal cerebral ischemia as protect the neurons from apoptosis and the decreasing of PKCγ expression in rats brain.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第8期1006-1010,共5页
Chinese Pharmacological Bulletin
关键词
二异丙酚
脑缺血再灌注
凋亡
蛋白激酶C
propofol
brain ischemia and reperfusion
apoptosis
protein kinase C
