摘要
目的 观察延髓和脊髓背角内蛋白激酶Cγ亚单位 (PKCγ)样阳性神经元向中脑导水管周围灰质(PAG)的投射。 方法 荧光金 (FG)逆行追踪与PKCγ的免疫荧光组织化学染色相结合的双标记技术。 结果 PKCγ样阳性神经元主要分布于延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核 ;将FG注入PAG后 ,在延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核内可见FG标记神经元 ;部分FG标记神经元呈PKCγ样阳性 ,FG PKCγ双标神经元也主要见于延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核。 结论 延髓和脊髓背角的PKCγ阳性神经元可能参与将伤害性刺激信息向PAG的传递。
Objective To examine the projection of protein kinase Cγ isoform(PKCγ)\|immunoreactive neurons from the medullary and spinal dorsal horns to the midbrain periaqueductal gray(PAG) in the rat. Methods By using fluoro\|gold(FG) retrograde tracing combined with immunofluorescence histochemical staining for PKCγ. Results PKCγ\|immunoreactive neurons were observed in laminae Ⅰ,Ⅱ,Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus.After injecting FG into the PAG,FG retrogradely labeled neurons were also mainly found in laminae Ⅰ,Ⅱ,Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus.Some of these FG\|labeled neurons in laminae Ⅰ,Ⅱ,Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus exhibited PKCγ\|immunoreactivities.Conclusion\ PKCγ\|immunoreactive neurons in the medullary and spinal dorsal horns might be involved in the transmission of nociceptive information from the medullary and spinal dorsal horns to the PAG.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第1期6-10,共5页
Acta Anatomica Sinica
基金
国家自然科学基金 (3 9970 2 3 9
3 980 0 0 3 4
3 980 0 0 44 )
国家重点基础研究规划"脑功能和脑重大疾病的基础研究"(G19990 5 40 0 4)
高等院校骨干教师资助计划资助项目
