摘要
目的 观察延髓和脊髓背角内蛋白激酶 Cγ亚单位(PKCγ)阳性神经元向孤束核的投射 .方法 荧光金 (FG)逆行追踪与 PKCγ的免疫荧光组织化学染色相结合的双标记技术 .结果 PKCγ阳性神经元主要分布于延髓和脊髓背角的 , , 层及脊髓的外侧脊核 ;将 FG注入孤束核后 ,在延髓和脊髓背角的 , , 层及脊髓的外侧脊核内可见 FG标记神经元 ;部分 FG标记神经元呈 PKCγ阳性 ,FG/ PKCγ双标神经元也主要见于延髓和脊髓背角的 , , 层及脊髓的外侧脊核 .结论 延髓和脊髓背角的
AIM To examine the projection of protein kinase Cγ isoform (PKCγ) immunoreactive neurons from the medullary and spinal dorsal horns to the solitary tract nucleus in the rat. METHODS Fluoro gold (FG) retrograde tracing combined with immunofluorescence histochemical staining for PKCγ was used. RESULTS PKCγ immunoreactive neurons were observed in laminae Ⅰ, Ⅱ, Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus. After injecting FG into the solitary tract nucleus, FG retrogradely labeled neurons were also found mainly in laminae Ⅰ, Ⅱ, Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus. Some of these FG labeled neurons in laminae Ⅰ, Ⅱ, Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus exhibited PKCγ immunoreactivities. CONCLUSION PKCγ immunoreactive neurons in the medullary and spinal dorsal horns might be involved in the transmission of somatic and visceral noxious stimulus information from the medullary and spinal dorsal horns to the solitary tract nucleus.
出处
《第四军医大学学报》
北大核心
2001年第23期2171-2174,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 ( 39970 2 39
3980 0 0 44)
高等学校骨干教师资助计划资助项目
