摘要
本文对碱性蛋白酶Alcalase AF 2.4L水解玉米蛋白的动力学特征进行了较为系统的研究:(1)探讨固液比、酶量、温度、pH值等因素对玉米蛋白水解度的影响,研究确定了碱性蛋白酶Alcalase AF 2.4L水解玉米蛋白的最佳工艺控制条件;(2)根据所建立的pH-stat酶解体系确定了Vmax和Km,为更有效地利用玉米蛋白资源奠定了理论基础。
This paper has probed the dynamics of hydrolysis on CGM by Alcalase AF 2.4L: (1) The factors including the ratioof solid to liquid ,adding amount of enzyme,temperature of reaction ,pH value and time of reaction affecting the degree ofhydrolysis of CGM by Alcalase AF 2.4L were studied through single-factor-analysis; (2) The optimal conditions, the maximumvelocity and instant Km were determined through probing into the relation of the ratio of solid to liquid and enzyme reactionvelocity.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第10期29-32,共4页
Food Science
基金
国家星火计划项目(2001EA610021)
