摘要
以番茄浆为原料,利用纤维素酶与果胶酶辅助提取番茄红素。对提取过程中纤维素酶与果胶酶的比例及酶用量、酶解时间、酶解体系温度和pH等条件进行探讨,并通过正交试验得出最佳提取工艺条件。表明在果胶酶和纤维素酶质量比为3∶1、混合酶量0.12 g/20 g番茄浆、酶解温度45℃、pH 6.0、酶解时间6 h条件下,提取率最高,可达到23.03μg/g。
To obtain the optimized process parameter of extracting the lycopene from tomato pulp, single factor experiment and orthogonal test were adopted. The effect of compoud enzymes mixture ratio （pectinase/cellulase）, pH, time and temperature of enzymatic hydrolysi on the extraction rate of lycopene were studied. The results showed that compound enzyme best ratio was 3 ： 1, their total dosage 0.12 g/20 g and pH 6.0 for 6 h hydrolysis at 45 ℃, the extraction rate of pulp reached 23.03 μg/g.
出处
《食品工业》
北大核心
2013年第12期60-63,共4页
The Food Industry
基金
江苏省大学生实践创新训练计划(项目编号:2012JSSPITP3013)
关键词
番茄红素
果胶酶
纤维素酶
复合酶法
lycopene
cellulose
pecfinase
compound enzymes method
