摘要
以淀粉厂副产物玉米黄粉为原料,经超临界CO2萃取脱脂脱杂后提取玉米醇溶蛋白,将玉米醇溶蛋白酶解制取玉米寡肽。采用正交试验法确定出最佳超临界CO2萃取工艺参数,即萃取压力25MPa,萃取温度45℃,萃取时间100min,CO2流量30L/h,此时脂类及异杂味物质萃取率为8.06%,萃取后玉米黄粉蛋白质含量为90.46%、脂肪含量0.1%,与未经超临界CO2萃取处理及溶剂处理的玉米黄粉相比,玉米醇溶蛋白提取率分别提高50.33%、35.69%,玉米醇溶蛋白水解率分别提高47.16%、36.75%。
In this research, corn yellow powder is by-product of starch factory, using corn yellow powder as material to extract zein after supercritical CO2 fluid extraction, then manufacture oligopeptides using this zein through enzymolysis. The optimum technique condition of supercritical CO2 fluid extraction was determined through orthogonal test. When supercritical CO2 fluid extraction, the pressure is 25 MPa, temperature is 45℃, time is 100 min, CO2 flow is 30 L/h. percentage extraction is 8.06%, after extracting the content of protein is 90.46%, fat is 0.1%. Compare with non- supercritical CO2 fluid extraction and treatment by organic solvent, zein percentage extraction of corn yellow powder is more than 50.33% and 35.69%. Manufacturing oligopeptides using higher purity zein through enzymolysis, hydrolytic degree of zein more than 47.16% and 36.75% compare withl non-supercritical CO2 fluid extraction and treatment by organic solvent.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第8期166-170,共5页
Food Science
基金
长春市重大科技攻关项目(02-113G05)
