摘要
目的 初步探讨蚯蚓与日本血吸虫间共同抗原。方法 用十二烷基硫酸钠 -聚丙烯酰胺凝胶电泳分析蚯蚓抗原 (L t Ag)和日本血吸虫成虫抗原 (Sj Ag)的蛋白组分 ;并将其蛋白组分分别与日本血吸虫病患者阳性血清和兔抗蚯蚓的免疫血清进行斑点酶联免疫吸附试验、免疫印迹试验。结果 L t Ag和 Sj Ag的蛋白组分间存在较多分子量相近的蛋白条带。L t Ag抗原分子量在 39～ 90 k Da范围内与日本血吸虫病患者血清的交叉反应尤其明显。Sj Ag也能与兔抗蚯蚓血清较好的结合。结论 实验结果提示 ,L t Ag与 Sj Ag之间存在着至少
Objective To study preliminar il y the mutual antigens between Lumbricus terrestris[WT5”BZ ] and Schistosoma japonicum. Methods The antigen s of Lumbricus terrestris (L.terrestris) and [ WT5”BX]Schistosoma japonicu m (S.japonicum) were analysed by SDS-PAGE technique, and reacte d with the sera of patients with schistosomiasis and the sera of rabbits immunized with LtAg by Dot-ELISA and Western-blot. Results [WT5” B Z]There were many similar molecular weight antigens between LtAg and SjAg. Spe cially some antigens between 30-90 kDa form LtAg had intensive cross reactio n with the sera of patients with schistosomiasis. SjAg was also reacted with the sera of r abbits immunized with LtAg. Conclusion There are at least three immunocompetent antigens with similar molecular weight between L.terrestris and S.japonicum. [
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2004年第5期355-358,共4页
Chinese Journal of Schistosomiasis Control
关键词
蚯蚓
日本血吸虫
共同抗原
免疫活性
Lumbricus terrestris
Schistosoma japonicum
Mutual antigens
Immunocompetence
