摘要
以２０条／只和１００条／只剂量的尾蚴感染家兔，并于第９ｗｋ用吡喹酮对约半数家兔进行一次性灌胃治疗，依此建立日本血吸虫感染模型。用分别定位于日本血吸虫表皮膜、肠道上皮和虫卵的单克隆抗体建立直接斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）并应用该法分别检测感染家兔血中３类循环抗原、膜相关抗原（ＭＡＡ），肠相关抗原（ＧＡＡ）和可溶性虫卵抗原（ＳＥＡ），观察循环抗原滴度水平和日本血吸虫感染度之间的关系。结果发现，当用Ｄｏｔ－ＥＬＩＳＡ法检测３类循环抗原时，２０条尾蚴感染组与１００条尾蚴感染组家兔不论治疗与否，血中３类循环坑原滴度水平无显著性差异。
Halves of two groups of rabbits infected separately with two doses of Schistosoma japonicum(each rabbit infected with 20 or 100 cercariae) were treated with praziquentel(220 mg/kg body weight given as a single dose via stomach)at the 9th week after infection. In order to analyse the relationship between titers of circulating antigens(CAg) and infectiosity, we developed the DotELISA based on the application of 3 McAbs to detect respectively the gutassociated antigen(GAA), membrane associated antigen(MAA) and solube egg antigen(SEA) of Schistosoma japonicum. The results showed that the titers of CAgs in sera between the two groups of rabbits had no significant difference, whether they were treated or not. Therefore,DotELISA to detect CAgs is not suitable for judgement of infectiosity of Schistosoma japonicum.
出处
《中国寄生虫病防治杂志》
CSCD
1998年第3期204-206,共3页
Chinese Journal of Parasitic Disease Control
基金
联合国开发计划署
世界银行及WHO热带病研究和培训特别规划的部分资助
