摘要
【目的】探讨三七总皂甙(tPNS)对成年大鼠视网膜节细胞(RGCs)再生的影响。【方法】成年SD大鼠近端切断视神经(ON)并缝接自体一段坐骨神经(AG),腹腔注射tPNS。动物随机分为AG+溶剂(生理盐水)组;AG+tPNS组;量效关系组。用粒蓝逆行标记再生的RGCs,在荧光镜下观察视网膜平铺片再生RGCs的数量变化。【结果】①AG+溶剂组术后3和4周,每个视网膜再生的节细胞数分别为698±111和568±107;②在上述两个时间点AG+tPNS组每个视网膜再生的节细胞数分别为1656±135和1329±144约为对照组的2倍,P<0.05。【结论】三七总皂甙可促进视网膜节细胞再生。
To investigate the effects of total panax notoginseng saponins(tPNS) on regeneration of axotomized retinal ganglion cells (RGCs) in adults rats. After optic nerve (ON)transection, an autologous sciatic nerve(attached graft,AG)was removed and sutured to the proximal stump of the ON. tPNS was injected into abdominal cavity. Animals were divided into 3 groups: regenerating control group (AG+Solution); AG+tPNS group; effect and dosage group. Regenerating RGCs were labeled retrogradely by granulal blue, and the changes of regenerating RGCs in each retina were observed under fluorescent microscope. ①In control retinas, the mean number of regenerating RGCs were 698±111/retina and 568±107/retina respectively at the 3rd and 4th week after surgery. ②The mean number of regenerating RGCs in AG+tPNS group at the 3rd and 4th week were 1 656±135/retina and 1 329±144/retina, respectively,almost doubled when compared with control, P< 0.05. [Conclusion]tPNS could promote the regeneration of RGCs after axotomy.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2004年第4期319-321,共3页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家自然科学基金资助项目(39870266)
