摘要
目的探讨三七总皂甙(total spaonins of panax notoginseng,tPNS)对凝血酶诱大鼠血管平滑肌细胞(Vascular smoothmuscle cells,VSMCs)的促增殖作用的量效和时效关系。方法采用4～10代的VSMCs的单细胞悬液用于实验。通过MTS法和β-N-乙酰氨基己糖苷酶测定VSMCs细胞增殖率,观察tPNS对凝血酶诱大鼠VSMCs增殖的影响。结果(1)0.5u/ml和1.0u/ml两种浓度的凝血酶均有促VSMCs增殖作用(P<0.05)。增殖曲线呈双峰样改变(峰值在1h和24h);(2)不同tPNS浓度(300.0～600.0μg/ml)在24h时对0.5u/ml凝血酶刺激VSMCs都有明显抑制作用,且各浓度之间有显著性差异(P<0.05);(3)相对凝血酶组,tPNS(450.0μg/ml)有效地降低了凝血酶(0.5u/ml)诱导VSMCs增殖的1h峰值及24h峰值(P<0.05)。结论(1)凝血酶促VSMCs的增殖在时间上呈双峰样改变;(2)不同浓度的tPNS对凝血酶诱大鼠血管平滑肌细胞增殖均有抑制作用;(3)tPNS不仅能抑制凝血酶早期快速促VSMCs增殖作用,而且能抑制凝血酶后期促VSMCs增殖作用。
Objective To study the effect of total spaonins panax notoginseng （tPNS）in thrombin - induced Vascular Smooth Muscle Cells （VSMCs） proliferation.Methods Growth-arrested VSMCs （4- 10 generation ） were used in experiment. Cell proliferation was quantified by MTS or hexosaminidase activity method.Results （1）Compared with control group ,cell proliferation was significant- ly induced by thrombin （0.5u/ml and lu/ml） at selective time （1,6,12,24,48,72h）（P〈0.05）,and two proliferation curves were similar （P〉0.05）. The proliferation curve had two peaks,one at 1 hour ,and the other at 24h. （2）tPNS at various concentrations （300.0- 600.0μg/ml） significantly decreased thrombin-induced VSMCs proliferation at 24h （P〈0.05）,and the effects of various concentrations of tPNS （300.0~600.0μg/ml） were different to each other （P〈0.05）. （3）Compared with thromhin group, tPNS （450.0μg/ml）decreased the thrombin-induced cells proliferations at lh（P〈0.05） and at 24h（P〈0.05）. Conclusion （1）The cell proliferation induced by thrombin has two peaks at 1 hr and 24hr. （2）tPNS at various concentrations significantly decreased thrombin-induced VSMCs proliferation .（3 ）The inhibition effect of tPNS in thrombin - induced VSMCs proliferation was not only quickly and transient but also torpid and late.
出处
《江西医药》
CAS
2010年第1期11-14,共4页
Jiangxi Medical Journal
基金
2006年江西省卫生厅科研课题(项目编号20063138)
关键词
凝血酶
血管平滑肌细胞
三七总皂甙
增殖
thrombin
vascular smooth muscle cells
total spaonins panax notoginseng
proliferation
