摘要
目的 探讨霍乱毒素 (CTX)对金黄地鼠视网膜节细胞 (RGCs)再生的促进作用。 方法 成年金黄地鼠近端切断视神经 (ON )并缝接一段自体坐骨神经 (AG) ,玻璃体内注射 CTX 及 /或插入小段坐骨神经分支 (SN)。动物随机分为 AG组和溶剂组 ;AG+ CTX组 ;AG+ SN组 ;AG+ CTX+ SN组 ;量效关系组。前 4组动物存活 2～ 6周。用粒蓝逆行标记再生的 RGCs,在荧光镜下观察视网膜平铺片再生 RGCs的数量变化。 结果 AG+ CTX 组各时间点视网膜再生 RGCs平均数比 AG组和溶剂组增加 ,具有统计学意义 (P<0 .0 5 ) ;AG+ SN组也得出相似结果。AG+ CTX+ SN组各时间点的视网膜再生 RGCs平均数分别比 AG组和 AG+ SN组明显增加 (P<0 .0 1)。 结论 提示霍乱毒素或 /与坐骨神经具有显著促进视神经切断后视网膜节细胞再生的作用。
Objective The present study was aimed at investigating the effects of cholera toxin(CTx) on promoting the regeneration of retinal ganglion cells(RGCs) in hamster retina. Methods After optic nerve (ON) transection, an autologus sciatic nerve (attached graft, AG) was removed and sutured to the proximal stump of the ON. CT X was injected or (and) a small segment of sciatic nerve (SN) inserted intravitrously. Animals were separated into 5 groups:regenerating control group(AG groups and solution groups); AG+CT X groups; AG+SN groups; AG+CT X+SN groups; Effect and Dosage groups; Animals in the former 4 groups were allowed to survive for 2-6 weeks respectively. The regenerated RGCs were labeled retrogradely by granular blue, and the changes in number of regenerating RGCs in each retina were observed under fluorescent microscope. Results The mean numbers of regenerating RGCs in AG+CT X groups were increased and significantly higher than those in AG groups and solution groups at each time point( P <0 05). Similar results were obtained in AG+SN groups; In AG+CT X+SN groups, the mean number of regenerating RGCs was markedly increased than those in the AG groups and AG+SN groups at each time point ( P <0 01). Conclusion Our results indicated that injection of CT X or/and insertion of SN intravitrously could enhance the regeneration of the axotomized RGCs significantly.;
出处
《解剖学报》
CAS
CSCD
北大核心
2001年第2期117-120,T004,共5页
Acta Anatomica Sinica
基金
国家自然科学基金!资助项目 (39870 2 6 6 )
广东省自然科学基金!资助项目 (980 0 96 )
