摘要
本文用图像分析的方法对正常胃粘膜、轻、中、重度异型增生、管状腺癌及低分化腺癌共30例石蜡切片标本的细胞核仁组成区嗜银蛋白(AgNOR)和细胞核DNA含量进行了定量检测,并进行了两者的相关性研究。结果显示AgNOR颗粒数量及DNA含量均随病变程度加重而增加,AgNOR数量与DNA含量、超二倍体及超四倍体细胞百分数、DNA直方图倍体类型之间有明显的相关性(r=0.8811,0.7288,0.8606,0.8793);提示AgNOR数量与DNA含量的变化对胃癌前病变的判断与研究具有相近的意义。与细胞核DNA含量检测相比,AgNOR计数分析具有染色简便,易于推广的优点,是胃癌及癌前病变研究中一项新的、实用的参考指标。
The nucleolar organizer region associated protein (AgNOR) andthe DNA content were measured by image analysis technique in paraffin sections of 30 cases with normal mucosa, (mild, moderate or severe dysplasia), adenocarcinoma or poorly differentiated adenocarcinoma. The results of study showed that AgNOR sites, DNA content, the percentage of >2c and >4c cells incerased with severity of transfro-mation from dysplasia to carinoma. There was a very good linear correlation between the mean numbers of AgNOR sites per nucleus and the assessment of gastric cancer and dysplasia.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1993年第1期26-29,共4页
Chinese Journal of Cancer
关键词
核仁组成区
胃肿瘤
嗜银蛋白
Dysplasia Nucleolar orgsnizer region DNA content Image analysis technique
