摘要
探讨了致死性中子辐射后重组人白介素11(Recombinanthumaninterleukin?11,rhIL?11)对小鼠骨髓损伤的防治作用及其机制。采用二级雄性BALB/c小鼠96只,分别于4.0Gy中子全身照射前3d或后3d皮下注射rhIL?11,于照射后6h、1d和2d活杀取材,计数外周血和骨髓有核细胞。采用核仁组成区嗜银蛋白(ArgyrophilicofNucleaolarorganizerregions,AgNOR)染色检测骨髓细胞核嗜银蛋白含量、流式细胞术和DNA凝胶电泳检测骨髓细胞凋亡、免疫组化染色检测IL?11受体α及gp130的表达。结果表明,照射前应用IL?11对4.0Gy中子,照射后小鼠外周血白细胞和血小板降低有一定抑制作用,而照射后应用IL?11则对白细胞、红细胞、血小板以及骨髓有核细胞降低均有一定抑制作用;照射前及照射后用药组对细胞凋亡未见明显影响,而与单纯对照组比较,用药组、AgNOR含量增多,IL?11Rα及gp130表达均明显增强。说明IL?11可能通过受体α及gp130信号通路,促进损伤后造血功能恢复,从而发挥其对骨髓辐射损伤的防治作用。
In order to study the effects of rhlL-11 on radiation gamages to bone marrow cells in mice induced by 4.0Gy neutron irradiation, 96 BALB/c male mice were treated with rhIL-11 by subcutaneous injection 3 d before or after 4.0Gy total body irradiation （TBI） with neutrons. The peripheral blood ceUs and bone marrow nuclear cells were counted. The proliferation and apoptosis of bone marrow cells were evaluated by AgNOR staining, flow cytometry and DNA gel electrophoresis, respectively. The expressions of IL-11 Roand gp130 were studied by immunocytochemistry and image analysis. After the irradiation, the peripheral blood cells, content of AgNOR and expressions of IL-11Rα and gp130 decreased obviously. However, the trends of WBC, PLT were suppressed in the group treated with rhIL-11 before the irradiation, and the trends of WBC, RBC, PLT were suppressed in the group treat with rhIL-11 after the irradiation. In both the treated groups, the content of AgNOR and expressions of IL-11Rα and gp130 are higher than those in control group. But there was not significant difference in apoptosis. IL-11 may play a positive role against injuries to bone marrow by promoting the reconstruction of bone marrow after the irradiation through IL-11Rα and gp130 signaling.
出处
《辐射研究与辐射工艺学报》
EI
CAS
CSCD
北大核心
2006年第1期47-52,共6页
Journal of Radiation Research and Radiation Processing
基金
全军"十五"指令性课题(01L019)
国家自然科学基金(30370438)资助
