摘要
目的 :研究银杏叶提取物 (GBE)对大鼠局灶性脑缺血再灌注后SOD、MDA及NO含量的影响 ,探讨GBE的脑保护作用的机制。方法 :4 0只Wistar雄性大鼠随机分为假手术组 (A)、缺血组 (B)、小剂量GBE组 (C)、大剂量GBE组 (D)。制造大鼠大脑中动脉缺血再灌注模型。C、D组于术前 30min及术后 1h分别给予银杏叶提取物2 0mg/Kg、4 0mg/Kg腹腔内注射。应用TTC染色观察梗死体积、检测脑缺血组织SOD活性、MDA和NO含量。结果 :①SOD活性C、D组明显高于B组 (P <0 .0 1) ,D组高于C组 (P <0 .0 5 )。②MDA和NO含量C、D组明显低于B组(P <0 .0 1) ,D组低于C组 (P <0 .0 5 )。③梗死体积C、D组明显小于B组 (P <0 .0 1) ,D组小于C组 (P <0 .0 5 )。结论 :银杏叶提取物可减少脑缺血再灌注后自由基生成及梗死体积 ,疗效与剂量有关。
Objective: To study the protective effects of Ginkgo biloba extract(GBE) on SOD, MDA and NO in rats following focal cerebral ischemia and reperfusion.Methods: Forty Wistar male rats were randomly divided into 4 groups: A (sham-operated group); B (ischemic group); C (small-dose GBE treatment group); D (high-dose GBE treatment group). The model of middle cerebral artery occlusion (MCAO) and reperfusion was set up. GBE of 20 mg/kg and 40 mg/kg was respectively intraperitoneally injected into the rats 0.5 h before and 1 h after focal cerebral ischemia. The infarct volumes were quantified by TTC staining. The levels of SOD, MDA and NO were measured. Results: ①The levels of SOD in C and D groups were significantly higher than in B group ( P <0.01) and the level of SOD in D group was higher than in C group ( P <0.05).② The levels of MDA and NO in C and D groups were significantly lower than in B group ( P <0.01) and the levels of MDA and NO in D group were lower than in C group ( P < 0.05). ③ The infarct volumes in C and D groups were less than in B group ( P <0.01), and the infarct volume in D group was less than in C group ( P <0.05). Conclusion: GBE can decrease the production of free radicals and infarct volumes following cerebral ischemia and reperfusion in a dose-dependent manner.
出处
《中国康复》
2004年第2期73-74,共2页
Chinese Journal of Rehabilitation
