期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

人抵抗素基因cDNA的克隆 被引量：5

cDNA cloning of complete resistin gene coding sequence from Chinese
暂未订购
导出
摘要 目的　克隆人抵抗素基因 (hRETN)cDNA ,为进一步研究RETN的结构和功能提供实验基础。方法　应用RT PCR方法从中国人网膜脂肪垫总RNA中扩增出RETN基因cDNA ,克隆入载体 pMD18 T中 ,形成重组载体 pMD18 T/hRETN。通过蓝白斑筛选出阳性克隆 ,限制性内切酶酶切鉴定后对其进行测序。结果　从脂肪组织总RNA中扩增得到 36 3bp片段hRETN基因 ,其cDNA序列与GenbankhRETN基因序列基本相同。结论　成功地克隆中国人hRETNcDNA。 Objective To provide experimental basis for further research on resistin function,human resistin gene (hRETN) was cloned. Methods hRETN mRNA from omentum fat pat of Chinese was amplified by RT-PCR and cloned into plasmid pMD18-T to generate the recombinant plasmid pMD18-T/hRETN,which was confirmed to contain the amplified target gene segments by digest of restrictive endonuclease and nucleotide sequencing. Results The sequencing results for amplified target gene showed that the sequence of hRETN from omentum fat pat of Chinese was identical to that of human RETN in Genbank. Conclusion hRETN cDNA is successfully cloned from Chinese.
作者 周剑 王佑民 王长江 章秋 胡红琳 时照明
机构地区 安徽医科大学第一附属医院内分泌科
出处 《安徽医科大学学报》 CAS 2004年第2期119-122,共4页 Acta Universitatis Medicinalis Anhui
基金 安徽省教育厅自然科学资助项目 (编号 :2 0 0 2KJ15 8)
关键词 人抵抗素基因 CDNA 克隆 脂肪组织 氨基酸序列 adipose tissue/metabolism clone,molecular amino acid sequence DNA,complementary
分类号 R589.2 [医药卫生—内分泌]
  • 相关文献

参考文献9

  • 1Steppan CM, Bailey ST, Bhat S et al. The hormone resistin links obesity to diabetes . Nature, 2001 ;409(6818):307～12
  • 2Kim KH,Lee K, Moon Y S et al. A cysteine-rich adipose tissue-pecific secretory factor adipocyte differentiation. J Biol Chem, 2001; 276(14):11252～6
  • 3Patel L, Buckels AC, Kinghorn-IJ et al. Resistin is expressed in human macrophages and directly regulate by PPAR gamma activators. Biochem Biopys Res Commun, 2003;300(2):472～6
  • 4Raghu P, Ghosh S, Soundarya K et al. Dimerization of human recombinant resistin involves covalent and noncovalent interactions. Biochem Biophys Res-Commun. 2004;313(3):642～6
  • 5Savage DB, Sewter CP, Klenk ES et al. Resistin/FIZZ3 expression in relation to obesity and peroxisome prolifeator-activated receptor-gamma action in humans. Diabetes, 2001;50(10):2199～202
  • 6McTernan CL. McTernan PG.Herte AL et al. Resistin,central obesity ,and diabtes.Lancet, 2002;359(9300):46～7
  • 7WAY JM, Gorgun CZ, Tong Q et al. Adipose tissue resistin expression is severely suppressed in obrsity and stimulate by PPARγ agonist.-J Biol Chem, 2001;276(28):25651～3
  • 8Nagaev I ,Smith U. Insulin resiatance and type 2 diabetes are not relatsed to resistin expression in human fat cells or skeletal muscles. Biochem Biophys Res Commun, 2001;285(13):561～4
  • 9White BA. PCR Cloning Protool. New Jersey: Human Press,1997:61～110

同被引文献46

  • 1肖沪生,徐智章,张爱宏,银浩强,章怡祎,汪青良,彭欣.eTRACKING技术的原理及参数探讨[J].上海医学影像,2006,15(2):84-86. 被引量：72
  • 2孟庆玲,才学鹏,乔军,景志忠,张艳,田广孚,郭爱疆.中国白兔白介素-10基因的克隆、表达及其抗体的制备[J].中国实验动物学报,2007,15(3):203-207. 被引量：7
  • 3Steppan CM, Lazar MA. Resistin and obesity-associated insulin resistance [ J ]. Trends Eudocrinol Metab, 2002, 13 ( 1 ) : 18.
  • 4Banerjee RR, Lazar MA. Dimerization of resistin and resistin-like molecules is determined by a single cysteine [ J ]. J Biol Chem, 2001, 276(28) :25970.
  • 5Steppan CM, Bailey ST, Bhat S, et al. The hormone resistin links obesity to diabetes [ J]. Nature, 2001,409(6818) :307 - 312.
  • 6Shuldiner AR, Yang R, Gong DW. Resistin, obesity and insulin resistance--the emerging role of the adipocyte as an endocrine organ [J]. NEnglJMed, 2001, 345(18) :1345-1346.
  • 7Steppan CM, Brown E J, Wright CM, et al. A family of tissuespecific resistin-like molecules [ J]. Proc Natl Acad Sci USA, 2001, 98(2) :502 -506.
  • 8Senior K. Looking at the molecular explanation for obesity [ J]. Lancet, 2001, 357(9265):1344.
  • 9Morash BA, Willkinson D, UR E, et al. Resistin expression and regulation in mouse pituitary [ J]. FEBS Lett, 2002, 526 ( 1 - 3) :26 -30.
  • 10Kawashima J, Tsuruzoe K, Motoshima H, et al. Insulin down regulates resistin mRNA through the synthesis of protein(s) that could accelerate the degradation of resistin mRNA in 3T3-L1 adipoeytes [J ]. Diabetologia, 2003, 46 (2) :231 - 240.

引证文献5

二级引证文献11

安徽医科大学学报
2004年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部