摘要
我们用血友病FⅧ基因探针P114.12从人肝细胞cDNA文库中分离到3个有杂交信号的克隆。进一步鉴定,其中1个(FⅧhc_1)的克隆片段比所用的探针P114.12(0.65kb)增大0.45kb。通过FⅧhc_1部分顺序分析及微机搜索,FⅧhc_1有210bp与FⅧcDNA(5559-5769)完全同源,提示FⅧhc_1从FⅧ基因cDNA17号外显子向下游延伸了800～900bp。
A cDNA library prepared from human liver was screened for Factor Ⅶ,a clotting factor in the middle phase of blood coagulation,with P114.12 probe.3 recombinant phages were distinguished,and one of the 3recom-binants was further analyzed and extended by 450 bp as compared with the p114.12 probe.The clone 1 (FⅧhc1) was sequenced and homology between 210 bp of FⅧhc1 and 5559-5769 in FⅧ cDNA was found.It was shown that the FⅧhc1 extended forwardly 800~900bp from 17 exon of FⅧ cDNA.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1991年第4期349-352,共4页
Journal of Third Military Medical University
基金
复旦大学国家重点基因工程开放实验室资助项目
