摘要
目的:研究中国东北地区汉族人群抵抗素(RETN)-420C/G基因单核苷酸多态性(SNPs)的等位基因、基因型频率分布及其与2型糖尿病(T2DM)大血管病变之间的关系。方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对180例样本[T2DM并发大血管病变组60例,单纯T2DM组60例,正常对照(ND)组60例],进行基因分型及频率测定。结果:T2DM并发大血管病变组及单纯T2DM组等位基因频率及CC/GC+GG基因型分布与ND组比较差异均有统计学意义(P<0.01);T2DM并发大血管病变组等位基因频率及CC/GC+GG基因型分布与单纯T2DM组比较差异亦有统计学意义(P<0.05),T2DM并发大血管病变组GC+GG基因型频率明显高于单纯T2DM组。结论:RETN-420C/G基因多态性与中国东北地区汉族人群T2DM及其大血管病变的发生有相关性。
Objective To study the allelic and genotypic frequence distribution of RETN-420C/G single nucleotide polymorphisms（SNPs） and its relationship with type 2 diabetes mellitus（T2DM） complicated with macroangiopathy in Han population of northeast China.Methods The genotypes and their frequencies of a total of 180 cases,including 60 cases of T2DM complicated with macroangiopathy,60 cases of simple T2DM and 60 cases of normal control（ND）,were measured by PCR-RFLP.Results The allelic frequencies and simple CC/GC＋GG genotype in T2DM complicated with macroangiopathy group were significant different compared with ND group（P〈0.01）.The allelic frequencies and CC/GC＋GG genotype in T2DM comlicated with macroangiopathy group were significant different compared with T2DM group（P〈0.05）.The GC＋GG genotype in T2DM complicated with macroangiopathy group was significantly higher than that in T2DM group（P〈0.05）.Conclusion The RETN-420C/G polymorphism has a correlation with the occurrence of T2DM complicated with macroangiopathy in Han population of northeast China.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2011年第2期300-303,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划项目资助课题(200905196)
关键词
2型糖尿病
抵抗素
基因多态性
大血管病变
聚合酶链反应
type 2 diabetes mellites； resistin； gene polymorphism； macroangiopathy； polymerase chain reaction
