摘要
目的 :观察正常大鼠在高脂饮食诱导下形成肥胖后 ,胰岛素敏感性及其FFAs的变化。方法 :9周龄健康雄性Wistar大鼠16只 ,随机分为正常饲养组 (NC,n=8)和高脂饲养组 (HF,n=8)。喂养10周 ,比较两组大鼠体重、HOMA -IR以及FFAs等的变化。结果 :经10周高脂喂养 ,HF组与NC组比较 ,体重和内脏脂肪组织显著增加 (HF组大鼠体重较NC组增加了11 4% ,P<0 05) ;HF组大鼠内脏脂肪组织较NC组增加了20 7% ,P<0 01) ,并出现胰岛素抵抗 (HOMA -IR :HF组8 88±4 25vs3 92±2 26 ,P<0 05) ,空腹FFAs水平显著上升 ,较NC组增加了80 8% (P<0 05) ,但两组空腹血糖差异无显著性 ,HF组 :(7 89±1 46)mmol/LvsNC组 (8 70±1 59)mmol/L,P>0 05。结论 :高脂饮食可诱导大鼠肥胖伴胰岛素抵抗 。
Objective:To observe the changes of fasting FFAs and insulin sensitivity in obesity rats induced by high-fat diet.Methods:Normal male Wistar rats(9weeks old)were divided into two groups taking either norml chow(NC,3.3Kcal/g,15～20%calories for fat)or high-fat diet(HF,5.1Kcal/g,61%calories for fat).10weeks later,body weight,visceral fat mass,fasting glucose,fasting insulin and FFAs were de-termined,and HOMA-IR was calculated by the method HOMA-IR=FPG×FINS/22.5.Results:Rats of HF showed significantly that body weight and visceral fat mass gained(body weight increased by11.4%in HF compared with that in NC,P<0.05.Visceral fat mass increased by20.7%in HF compared with that in NC,P<0.01)and the insulin resistance occurred(HOMA-IR:HF:8.88±4.25vs NC:3.92±2.26,P<0.05)after10weeks feeding.The concertration of fasting plasma FFAs was also increased significantly in HF group compared with NC group by80.8%(P<0.05).But there was no difference in fasting plasma glucose in the two groups(HF:7.89±1.46mmol/Lvs NC:8.70±1.59mmol/L,P>0.05).Conclusion:In this study,obesity rat with insulin resistance was induced successfully with high-fat diet,and the state of insulin resistance of obesity rats had correlation with the increased concentration of fasting plasma FFAs.
出处
《现代医药卫生》
2004年第7期490-491,共2页
Journal of Modern Medicine & Health
