摘要
首次研究了吖啶橙 罗丹明B二聚体能量转移体系作为荧光探针用于DNA的测定 ,并对其机理进行了探讨。用于鲱鱼精DNA和小牛胸腺DNA的测定 ,线性范围分别为 0 33～ 1 33mg·L- 1 ,0 33～ 3 33mg·L- 1 ,检测限分别为 1 6 3× 10 - 3mg·L- 1 ,1 5 2× 10 - 3mg·L- 1 。对 1 0 0mg·L- 1 鲱鱼精DNA和小牛胸腺DNA的测定 ,相对标准偏差分别为 2 4 %和 2 0 %。
Not only dye monomer is the form of energy transfer but also dye dimer is the effective form of energy transfer. In this work, the energy transfer system of acridine orange(AO)-rhodamine B(RB) dimer was built for the determination of DNA as fluorescence probe. At the same time, the mechanism was also considered. Under the optimum experimental conditions, the linear range of this assay was 0.33-1.33 mg·L -1 for fish sperm DNA and 0.33-3.33 mg·L -1 for calf thymus DNA, the detection limit was 1.63×10 -3 mg·L -1 for fish sperm DNA and 1.52×10 -3 mg·L -1 for calf thymus DNA, respectively. The RSD for the determination of 1.00 mg·L -1 DNA was 2.40% for fsDNA and 2.00% for ctDNA, respectively. The method was used for the determination of DNA in synthetic samples with satisfied results.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第1期85-88,共4页
Spectroscopy and Spectral Analysis
基金
国家教育部骨干教师基金 (99JL0 79)资助项目
