摘要
目的：根据能量转移荧光猝灭程度,建立一种荧光猝灭法测定环境水样中痕量镉的新方法.方法：在无水乙醇介质中,Cd^2＋与1-（2-吡啶偶氮）-2-萘酚（PAN）形成PAN-Cd^2＋配合物,其吸收光谱与罗丹明6G（Rh6G）的发射光谱有效重叠,发生能量转移,使Rh6G荧光猝灭,从而建立了痕量镉的荧光猝灭测定新方法.结果：在优化实验条件下,在31～800 ng/ml浓度范围内,Rh6G荧光猝灭程度与PCd^2＋呈现良好的线性关系（r=0.9999）.方法的检出限为9.0 ng/ml.结论：方法灵敏、快速、简便,用于环境水样中痕量镉的测定,结果满意.
Objective：To set up a novel fluorescence quenching method for the determination of trace cadmium based on the energy transfer. Methods：In dehydrated alcohol, the fluorescence intensity of Rh6G was diminished greatly due to the energy transfer from Bh6G to complex PAN - Cd2 ＊. A novel fluorescence quenching method for the determination of trace cadmium was developed based on the Rh6G - PAN - Cd2 ＊ energy transfer system. Results： Under the optimal experimental conditions, the linear range of calibration curves for the determination of Cd2 was 31 - 800 ng/ml （ r = 0. 9999 ）. The detection limit was 9. 0 ng/ mL Conclusion ：The method has high sensitivity and simplicity. It had been satisfactorily used in the determination of trace cadmium in water samples.
出处
《中国卫生检验杂志》
CAS
2007年第8期1353-1354,1493,共3页
Chinese Journal of Health Laboratory Technology
关键词
镉
PAN
能量转移
荧光猝灭
罗丹明6G
Cadmium
PAN
Energy transfer
Fluorescence quenching
Rhodanmine 6G
