摘要
通过改进培养基的配方,控制2,4-D和蔗糖浓度以及培养时间,获得了龙眼胚性愈伤组织体胚发生各个阶段即球形胚、心形胚、鱼雷形胚、子叶形胚、成熟体胚等阶段高度同步化的胚性培养物;对龙眼胚性培养物进行了DNA、RNA提取方法研究,获得了完整、高质量的DNA和RNA,摸索出一套简便、快捷、有效的DNA提取方法,并对提取的RNA进行了反转录及PCR扩增试验,为龙眼体细胞胚胎发生的分子生物学研究奠定了重要基础。
Culture of embryo calli of Longan (Dimocarpus longcai Lour) on improved cultural media with controlled concentrations of 2,4-D and sucrose at different developmental time resulted in highly synchronized embryogenic cultures including globular embryoids, heart-shaped embryoids, torpedo-shaped embryoids, cyto-shaped embryoids and matured embryoids. High - quality DNA and RNA were extracted from embryogenic cultures. A simple, quick and effective method for extracting DNA from embryogenic culture was established. Moreover, further reverse transcription and PCR were conducted with the extracted RNA, which would be of great importance for the studies on molecular biology in longan.
出处
《热带作物学报》
CSCD
2003年第3期31-35,共5页
Chinese Journal of Tropical Crops
基金
国家教育部首批高等学校骨干教师资助计划(2000-65)
霍英东教育基金会高等学校青年教师基金(71026)
福建省自然科学基金(B0010014)的资助
