摘要
采用HPLC法测定了龙眼“红核子”品种胚性愈伤组织胚胎发生过程中各个不同发育阶段的内源激素含量变化。结果表明,龙眼胚性愈伤组织中内源激素含量比非胚性愈伤组织高;除GA3外,体胚发生过程中内源激素变化趋势大致呈“M”状:胚性愈伤组织Ⅱ和球形胚为变化的2个明显转折点;内源激素的变化先于形态学的变化。
The changes of the endogenous phytohormone content of synchronic materials at different developmental stages in somatic embryogenesis were measured by HPLC in Dimocarpus longan Lour. cv. Honghezi. The results showed that the levels of endogenous phytohormones in embryogenic calli were higher than those in non-embryogenic calli. Except for GA3, the changes of endogenous hormones in the process of somatic embryogenesis showed approximately a 'M' curve. Embryogenic Callus II and globular embryoids were the 2 turning-point stages in the changes of endogenous phytohormones in somatic embryogenesis. The changes of endogenous phyhormones were found earlier than those of morphogenesis.
出处
《热带作物学报》
CSCD
2002年第2期41-47,共7页
Chinese Journal of Tropical Crops
基金
本研究得到国家教育部首批高等学校骨干教师资助计划
国家自然科学(No.39870544)
福建省自然科学基金(No.B0010014)的资助
关键词
胚胎发生过程
龙眼
体细胞胚胎
内源激素
HPLC法
longan (Dimocarpus longan Lour.)
somatic embryogenesis
endogenous phytohormones
