摘要
比较了龙眼不同类型胚性愈伤组织、品种、蔗糖质量浓度、有机附加物、生长调节剂、光照度等因素对龙眼胚性愈伤组织体胚诱导的影响.结果表明,在优化的体胚发生条件下,选择适宜的胚性愈伤组织类型,可获得龙眼胚性愈伤组织的高频率体细胞胚胎发生.在低质量浓度蔗糖(≤30 g·L^(-1))和黑暗条件下,松散型胚性愈伤组织的体胚分化频率为100%,并且发生数量可达每克1万个左右.
The effects of types of calli,varieties,mass concentrations of sucrose,organic additions,plant growth regulators and illuminance on induction of somatic embryoids from longan embryogenic calli were copmared.Somatic embryogeneses of high frequency were induced from suitable types of embryogenic calli under the optimum conditions of induction of somatic embryoids.Strong embryogenetic capacity with 100% frequency of embryogeneses occured in friable embryogenic calli cultured on MS medium containing low mass concentrations of sucrose(≤30 g · L^(-1))in darkness,and each gram of calli could form some 10 000 embryoids.
出处
《福建农业大学学报》
CSCD
1997年第3期271-276,共6页
Journal of Fujian Agricultural University
基金
福建省科委"八.五"重点攻关项目(B-91-Z-14)
福建农业大学青年教师甲种基金
关键词
龙眼
胚性愈伤组织
体胚发生
高频率
longan
embryogenic callus
somatic embryogenesis
high frequency
