摘要
目的 探讨MMP - 2、CD4 4v6、端粒酶活性与宫颈癌的关系。方法 采用免疫组织化学S -P法和银染色端粒酶重复扩增法分别检测 6 2例宫颈癌对MMP - 2、CD4 4v6、端粒酶的表达情况并进行统计学分析。结果 正常宫颈组织对MMP - 2、CD4 4v6呈低表达 ,其端粒酶活性低。而在宫颈癌组织中MMP - 2、CD4 4v6均呈高表达 ,其端粒酶的活性增强。有淋巴结转移组其MMP - 2、CD4 4v6呈过高表达 ,端粒酶活性明显增强 ,与无淋巴结转移组比较有显著性差异。结论 MMP - 2、CD4 4v6、端粒酶的表达与宫颈癌有关。且MMP - 2、CD4 4v6、端粒酶阳性表达组织具有淋巴结转潜能。
Objective:To investigate the relationship between MMP-2,CD44v6, telomerase in cervical cancer and lymph nodemetastasis.Methods:Telomerase activity was measured by non-radioisotopic silver staining telomere repeat amplification protocal (TRAP). The expression of MMP-2 andCD44v6 was detected by the means of immunohistochemistry S-P in the cervical tissue of 20 cases with normal uterine cervix and 62 cases with cervical cancer.Results:In the normal cervical tissues there was not expression of MMP-2 and CD44v6, and the activity of telomerase was low, but in the cervical cancer the expression of MMP-2 and CD44v6 was high and the activity of telomerase was strong.In the group with lymph node metastasis MMP-2 CD44v6 was highly expressed.It was significantly different from the group without lymph node metastasis.Conclusions:MMP-2, CD44v6 and the activity of telomerase related with cervical cancer.The high expression of MMP-2,CD44v6 and the high activity of telomerase play important roles in lymph node metastasis.
出处
《中国现代医学杂志》
CAS
CSCD
2003年第20期21-24,共4页
China Journal of Modern Medicine
