摘要
目的 :用改良高效液相色谱法测定小鼠心肌腺苷酸含量。方法 :ODSC18柱 ,检测波长 2 5 4nm。甲醇 - 5 0mmol/L磷酸盐 (1∶99)缓冲液 (pH6 .0 7)为流动相。 结果 :心肌ATP、ADP和AMP平均回收率分别为 10 1.1%± 9.3%、94 .8%± 10 .6 %及 98.3%± 5 .7% ;日内RSD分别为 3.5 %、6 .9%和 4 .8% ;日间RSD分别为 6 .4 %、8.6 %及 8.2 %。结论 :该方法简便、快速、灵敏。采用该法发现病毒性心肌炎小鼠心肌ATP、ADP和AMP含量降低。
Objective:A method for the quantitative determination of adenosine phosphates of mouse myocardium by HPLC is described.Methods:A ODS C 18 column and a mobile phase of methol-50mmol·L -1 phosphate (1∶99)buffer solution (pH 6.07),with UV detector at 254nm were used.Results:The average recovery rates of ATP,ADP and AMP were 101.7%±9.3%,94.8%±10.6% and 98.3%±5.7% respectively;the RSDs of within-day were 3.5%,6.8% and 4.8%,and the RSDs of between-days were 6.4%,8.6% and 8.2%,respectively.Conclusion:The method is simple,rapid and senstive and can be used to analyse the adenosine phosphates in myocardium.The myocardial contents of ATP,ADP as well as AMP decline notably in mice with viral myocarditis.
出处
《重庆医科大学学报》
CAS
CSCD
2003年第4期485-486,490,共3页
Journal of Chongqing Medical University
关键词
高效液相色谱法
腺苷酸
High performance liquid chromatography(HPLC)
Adenosine phosphate
