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人参毛状根及愈伤组织中ATP动态变化的生物发光法测定 被引量:1

Bioluminescent determination of ATP dynamic changes in hairy root and cultured callus of Panax ginseng C. A. Mey
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摘要 ATP content of cultured callus and hairy root introducted by Ri plasmid of \%Panax ginseng \%C. A. Mey was detected by bioluminescence. The result showed that ATP content in hairy root is obviously higher than that in callus within a growth cycle. The highest ATP content in hairy root is 26.6×10\+\{-12\}mmol/g (fresh weight), while ATP in callus is 2.68×10\+\{-12\}mmol/g (fresh weight). Total saponin content in hairy root reaches 2.486% (dry weight), while the saponin in callus is 1.105% (dry weight). The hairy root inducted by Ri plasmid has vigorous ability in secondary metabolism for ginseng saponin synthesis. ATP content of cultured callus and hairy root introducted by Ri plasmid of \%Panax ginseng \%C. A. Mey was detected by bioluminescence. The result showed that ATP content in hairy root is obviously higher than that in callus within a growth cycle. The highest ATP content in hairy root is 26.6×10\+\{-12\}mmol/g (fresh weight), while ATP in callus is 2.68×10\+\{-12\}mmol/g (fresh weight). Total saponin content in hairy root reaches 2.486% (dry weight), while the saponin in callus is 1.105% (dry weight). The hairy root inducted by Ri plasmid has vigorous ability in secondary metabolism for ginseng saponin synthesis.
出处 《植物资源与环境学报》 CAS CSCD 2001年第2期61-62,共2页 Journal of Plant Resources and Environment
关键词 人参 毛状根 愈伤组织 ATP 生物发光法 药用植物 动态变化 Panax ginseng C. A. Mey hairy root callus ATP bioluminescence
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参考文献8

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