摘要
用荧光光谱和紫外 可见吸收光谱法研究了在模拟人体生理条件下 ,芦氟沙星与牛血清白蛋白的结合反应 ,发现芦氟沙星对牛血清白蛋白有较强的荧光猝灭作用 ,用Stern Volmer和Lineweaver Burk方程处理荧光猝灭数据 ,得到了反应的结合常数。
The binding reaction betweeen rufloxacin hydrochloride and bovine serum albumin(BSA) was studied on the conditions of simulant clay's physiology by using fluorescence spectra and ultraviolet-visible light absorption spectra. It is shown that rufloxacin hydrochloride has a powerful ability to quench the BSA's fluorescence. The fluorescence quenching data were analyzed according to Stern-Volmer equation and Lineweaver-Burk equation,and the binding constants, thermodynamic parameters and combined positions were obtained.
出处
《武汉大学学报（理学版）》
CAS
CSCD
北大核心
2003年第4期433-436,共4页
Journal of Wuhan University:Natural Science Edition
基金
国家自然科学基金资助项目 (2 9973 0 3 0 )
湖北省教育厅科学研究重点资助项目 (2 0 0 3A0 0 3 )
