摘要
从患败血症仔猪的肺脏、淋巴结组织中分离到1株猪繁殖障碍与呼吸道综合征病毒(Porcinereproductiveandrespiratorysyndromevirus,PRRSV);流行特征表现为仔猪出现严重的呼吸道症状;该病毒能在Marc-145细胞上生长,但不能在Vero、BHK21、RK等细胞上增殖;分离毒适应Marc-145细胞后,产生以皱缩、脱落、崩解为特征的细胞病变,分离毒Marc-145第4代的TCID50为10-6.725·0.1ml-1;ELISA抗体检测表明,病猪和分离毒接种仔猪体内含有PRRSVIgG、IgM抗体。
A local strain of PRRSV was isolated from lungs and lymph nodes of piglets suffered hematosepsis.The isolate was high virulent and the affected piglets display serious respiratory disorder.The isolate could be propogated in Marc\|145 cell and cause the cell crumble,wrinkle and breaking apart,but not do so in other cell lines.ELISA tests showed that PRRSV IgG,IgM antibody could be detected in the infectious pig and isolate\|inoculating piglets.
出处
《福建农业学报》
CAS
2003年第2期93-96,共4页
Fujian Journal of Agricultural Sciences
基金
福建省农业科学院预试研究课题。
