摘要
以流行性乙型脑炎(乙脑)病毒感染C6/36细胞制备的乙脑抗原,用于捕捉法ELISA检测病人血清或脑脊液中的乙脑IgM抗体,可早期快速诊断乙脑病人。检测了62份可疑乙脑病人的血清,並以血凝抑制法作对照。结果,血凝抑制法的阳性检出率为35％(其中包括IgM阴性IgG阳性的6例),而捕捉法ELISA IgM的检出率为46.6％。另有6例双份血清,血凝抑制法有4倍升高,临床确诊为乙脑,经本法检测IgM抗体全部阳性。用本法查病人脑脊液中的IgM抗体,阳性检出率达到88.8％。结果可靠,还可将乙脑与疱疹病毒脑炎区别开。
An IgM capture ELISA for rapid diagnosis of Japanese encephalitis (JE) was studied. The JE virus antigen was prepared by infected C6/36 cells. From the examination of 62 serum samples,IgM capture ELISA was 46.6% positive and hemagglutination inhibition test was 35% positive only. The IgM antibody was all positive in 6 patients with 4-fold rise of HI anti body against JEV in paired sera. It seems that IgM capture ELISA is more sensitive than HI test, Using IgM capture ELISA to detect the JE IgM antibody from cerebrospinal fluid (CSF) was more reliable for diagnosis of the disease than the detection IgM from serum. Because it is not influenced by inapparent infection of JE virus in which IgM could be detected in the serum also. Specific IgM antibody detection from CSF could easily to differentiate JE from Herpes simplex virus encephalitis.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第4期378-382,共5页
Chinese Journal of Virology
关键词
ELISA
乙型脑炎
早期诊断
IgM capture ELISA Japanese Encephalitis Early diagnosis
