摘要
以肠道病毒71型(EV71)感染Vero细胞制备的EV71抗原,用于IgM捕捉ELISA法检测类脊髓灰质炎(类脊灰)患者血清中EV71 IgM抗体,特异性高,敏感性及稳定性良好。154例脊髓灰质炎(脊灰)病毒IgM阴性的可疑脊灰患者血清中EV71 IgM抗体阳性检出率为14.9％(23/154)。病后第2天的标本即可测出EV71 IgM,5至12天者抗体滴度较高,病后第40天的标本尚可测出EV71 IgM。本法是EV71感染的早期快速诊断方法,可用于与脊灰的鉴别诊断。
An IgM capture ELISA ( MacELISA ) for detection of enterovirus 71 (EV7l)IgM antibody was developed, which, was more sensitive,more specific than neutralization test and more specific than EV71 IgG indirect ELISA.One of three poliomyelitis-like cases with acute and convalescent paired sera was EV71 IgM positive by MacELISA and showed 8-fold rise in EV71 IgG by indirect ELISA. 151 cases of poliomyelitis-like disease with single sera, which were poliovirus Ⅰ , Ⅱ , Ⅲ ( PV- I , PV- Ⅱ , PV-Ⅲ) IgM negative were tested by EV71 MacELISA and 22 cases of them were EV71 IgM positive. The EV71 IgM positive rate in the sera from clinically-diagnosed poliomyelitis was 7.42%( 23/310) .EV71 IgM can be detected in the sera of poliomyelitis-like disease on the second day after the onset of the illness and reached a peak on day 8-10, and then decreased untill day 40. EV71 MacELISA is a rapid,early and specific diagnostic method and can be used in the differential diagnosis between poliomyelitis-like disease and poliomyelitis.
出处
《病毒学报》
CAS
CSCD
北大核心
1992年第1期75-79,共5页
Chinese Journal of Virology
关键词
肠道病毒71型
类脊髓灰质炎
ELISA
Enterovirus 71 IgM antibody capture ELISA Poliomyelitis-like disease
