摘要
采用硫酸铵盐析、ＳｅｐｈａｄｅｘＧ２００柱层析和亲和层析技术从鸡血清中提纯ＩｇＭ。经ＳＤＳ－聚丙烯酰胺凝胶电泳鉴定，纯化的ＩｇＭ达到电泳纯，其重链分子量为８００００道尔顿，轻链分子量为２２５００道尔顿。在双向琼脂免疫扩散试验中，能与羊抗鸡ＩｇＭμ链抗血清产生一条清晰的免疫沉淀线。免疫鸡与非免疫鸡比较，发现前者血清中ＩｇＭ含量为后者的２倍以上。
Chicken IgM was purified from serum by a procedure of precipitation with saturated ammonium sulfate,chromatography with Sephadex G200 and affinity chromatography with goat anti-μchain of chicken IgM.The heavy and light chains of the chicken IgM had molecular weights of 80 and 22.5 k dsltons,respectively,in SDS-polyacrylamide gel electrophoresis(SDS-PAGE).An immunoprecipitation line was shown between the purified chicken IgM and the goat anti-μchain of chicken IgM in the agar immunodiffusion test.Comparing immunized chicken with non-immunized chicken,we found that the amount of IgM in the former sera was more than twice that in the latter.
出处
《江苏农业学报》
CSCD
1994年第2期33-36,共4页
Jiangsu Journal of Agricultural Sciences
