摘要
目的 :建立血浆中硝苯地平的高效液相色谱测定方法。方法 :取血浆 1.0ml,以尼群地平为内标 ,用乙酸乙酯及环己烷 (3 1)提取两次 ,有机相在5 5℃水浴中氮气吹干 ,残渣用流动相复溶后进样分析。结果 :本法在 5～ 2 0 0 μg·L-1范围内线性良好 ,相关系数r =0 .9999(n =6)。高、中、低三种浓度硝苯地平的回收率为 90 .13%～ 99.4 3% ,日内精密度为 1.4 3%～ 6.61% ,日间精密度为 3.85 %～8.79% ,硝苯地平和内标的平均绝对回收率分别为90 .5 8%和 91.5 9%。内源性物质对测定无干扰。结论 :本法灵敏度及特异性高 ,操作简便 。
AIM: To develop a HPLC method for the determination of nifedipine in plasma. METHODS: Nitrendipine was used as the internal standard and 1.0 ml lasma was extracted by n hexane ethylacetate (31) in a two step extraction. The organic layer was transferred to another tube and evaporated to dryness under a stream of nitrogen gas at 55 ℃. The residue was reconstituted with the mobile phase. RESULTS: the linearity was obtained from 5 to 200 μg·L -1 of nifedipine in plasma with a good correlation (r= 0.9999 , n=6). The recoveries of nifedipine were 90.13 % to 99.43 %, and the coefficients of variation of within day and between days were 1.43 % to 6.61 % and 3.85 % to 8.79 %, and the average absolute recoveries of nifedipine and internal standard were 90.58 % and 91.59 % for the low, middle and high concentration of check samples, respectively. Endogenous substance did not interfere in the analysis. CONCLUSION: This method is sensitive, specific and simple and suited for the nifedipine pharmacokinetic study.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2003年第2期180-182,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
