摘要
目的建立测定人血浆中帕珠沙星浓度的高效液相色谱法。方法血浆样品用10%高氯酸沉淀蛋白。色谱柱为Diamonsil C18柱(200mm×4.6mm,5μm),流动相为0.02mol·L-1磷酸二氢钠溶液(含三乙胺0.5%,用磷酸调至pH3.0)-乙腈(82∶18),流速为1.0mL·min-1,紫外检测波长245nm。结果血浆中内源性物质对样品测定无干扰。本方法线性范围为0.05～50μg·mL-1(r=0.9999),最低定量浓度为0.05μg·mL-1,提取回收率大于80%,方法回收率为100.6%～101.4%,日内、日间RSD均小于6%。结论本法简便、灵敏、准确,适用于帕珠沙星药动学的研究。
OBJECTIVE To establish art HPLC method for the determination of pazufloxacin in human plasma. METHODS Proteins in plasma samples were precipitated by 10% perchloric acid. Pazufloxacin was separated on a Diamonsil C18 column（200 mm ×4.6 mm,5 μm）with the mobile phase consisted of 0.02 mol·L^-1 sodium dihydrogen phosphate solution（contained 0. 5% triethylamine,pH was adjusted to 3.0 with phosphoric acid） - acetonitrile （82： 18） at a flow rate of 1.0 mL·min^-1. The UV detection wavelength was set at 245 nm. RESULTS The blank plasma did not interfere with the determination of the analytes. The linear range was of 0.05 - 50 μg·mL^-1 （ r = 0.999 9 ）. The limit of quantitation was 0.05 μg ·mL^-1. The method recovery was 100.6% -101.4%. Within-day and between-day RSD were less than 6%. CONCLUSION The method was simple,sensitive and accurate,which was suitable for the pharmacokinetic study of pazufloxacin.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2008年第5期428-431,共4页
Chinese Journal of Modern Applied Pharmacy
