摘要
目的:建立以反相高效液相色谱法测定恒河猴血浆中间尼索地平浓度的方法。方法:色谱柱为C18,流动相为甲醇-水(63∶37),柱温为30℃,流速为1.0ml/min,检测波长为236nm,灵敏度为0.05AUFS,内标为尼莫地平。结果:间尼索地平检测浓度在1.75～448μg/L范围内线性关系良好;平均提取回收率为84.1%(RSD=4.09%),平均方法回收率为98.7%(RSD=2.76%);处理后的血浆样品放置7d性质稳定,血浆样品反复冻融及低温保存性质稳定。结论:本方法灵敏度高、重现性好、操作简便,可用于恒河猴血浆中间尼索地平浓度的测定。
OBJECTIVE： To establish a RP-HPLC method for concentration determination of m nisoldipine （m-Nis） in the plasma of rhesus monkeys. METHODS： The analysis was performed on a C18 column at 30℃ with a mobile phase of methanol water （6a：37） at a flow rate of 1.0ml/min. The detection wavelength was 236nm. The sensitivity was 0.05AUFS and the internal standard was nimoldipine. RESULTS： The detectable concentration of m Nis showed good linearity in the range of 1.75-448μ g/ L. The average recovery of extraction was 84.1% （RSD = 4.09% ）, and the average recovery of method was 98.7% （RSD - 2.76%） . The processed plasma samples remained stable in quality after having been stored for 7 days, and the plasma remained stable in quality through repeated freeze thawing or stored at low temperature. CONCLUSION： The present method is highly sensitive and reproducible, as well as convenient, which can be used for concentration determination of m-Nis in the plasma of rhesus monkeys.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第2期101-103,共3页
China Pharmacy
