摘要
目的 探讨重组日本血吸虫铁蛋白 (r Sj Fer)粘膜免疫诱导小鼠抗血吸虫的保护性免疫。 方法 实验鼠分为 8组 ,每组 10只 ,包括 r Sj Fer、霍乱毒素 B亚单位 (CTB)和 r Sj Fer+CTB灌胃免疫组 ,以及 r Sj Fer、CTB和r Sj Fer+CTB滴鼻免疫组 ,PBS灌胃及滴鼻对照组。在第 3次免疫后 2 wk,经腹部感染日本血吸虫尾蚴 4 0± 1条 ,4 5d后解剖观察减虫率和减卵率。在免疫前和感染前尾静脉采血和取粪样 ,EL ISA检测血清 Ig A、Ig G及粪内 s Ig A水平。 结果 灌胃及滴鼻各组减虫率依次为 3.98%、3.77%、2 5 .5 7%及 7.6 9%、4 .5 0 %、33.35 % ,每克肝减卵率依次为3.76 %、2 .4 6 %、34.75 %及 4 .4 0 %、0 .0 6 %、6 0 .10 %。 结论 r Sj Fer粘膜免疫能诱导小鼠产生抗血吸虫的保护性免疫。
Objective To test the immune protection against challenge infection in mice vaccinated intranasally or intragastrically with recombinant Schistosoma japonicum (S.j) Ferritin (rSjFer). \ Methods\ Mice were divided into 8 groups each with 10 mice. They were immunized intragastically with rSjFer, CTB, rSjFer+CTB and intranasally with rSjFer, CTB and rSjFer+CTB respectively. PBS was used intragastically or intranasally as control groups. The mice were challenged with 40±1 S.j cercariae per mouse 2 wk after the third vaccinization. Forty\|five days later, mice were killed and perfused, and the adult worms and eggs were counted. Serum and fecal samples were obtained before the first \{immunization\} and the challenge infection. IgA and lgG in sera and sIgA in feces were detected by ELISA. \ Results \ The worm reduction rate was 3\^98%, 3\^77%, 25\^57% in the intragastric vaccination groups and 7\^59%, 4\^50%, 33\^35% in the intranasal vaccination groups respectively. The egg reduction rate was 3\^76%, 2\^46%, 34\^75% and 4\^40%, 0\^06%, 60\^10% respectively. \ Conclusion \ This study showed that a significant immune protection against \{Schistosoma\} japonicum infection was induced by mucosal (intranasal and intragastic ) vaccination with rSjFer.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2003年第1期37-41,共5页
Chinese Journal of Parasitology and Parasitic Diseases
基金
湖南省科委 (oojzy2 1 1 5)
TDR/ WHO资助 (ID980 2 68)~~
