摘要
目的 探讨重组日本血吸虫胞质氨基肽酶粘膜免疫诱导小鼠抗攻击感染的保护力。 方法 实验小鼠分成 4组 ,每组 11只 ,包括CTB、rSjGST2 6+CTB、rSjGST -CA +CTB滴鼻免疫组和PBS滴鼻免疫为对照组。在第 3次免疫后 2周 ,每只经腹部感染 (4 0± 1)条尾蚴。 45d宰杀动物 ,观察减虫率和减卵率。在初次免疫前和攻击感染前采集唾液和尾静脉采血 ,ELISA检测血清及唾液内特异抗体水平。 结果 血清特异IgA和IgG及唾液内sIgA水平升高。rSjGST -CA +CTB和rSjGST2 6+CTB滴鼻免疫组减虫率分别为 2 9 62 %和 3 1 5 5 %。每克肝减卵率分别为 45 0 0 %和63 3 4%。
To test the immune protection against challenge infection in mice vaccinated intranasally with recombinant schistosoma japonicum (S j) GST-cytosal aminopeptidese (rSjGST-CA) Mice for experiment of rSjGST-CA were divided into 4 groups each with 11 mice They were immunized intranasally with CTB,rSjGST26+CTB,rSjGST-CA+CTB respectively PBS was used intranasally as control groups The mice were challenged with 40±1 S j cercariae per mouse 2 week after the third vaccination Forty-five days later,mice were killed and perfused,and the adult worms and eggs were counted Serum and saliva samples were collected before the first immunization and the challenge infection IgA and IgG in sera and slgA in salvia were detected by ELISA Results showed that the level of IgA and IgG in sera and slgA in salvia of mice increased after immunization The worm reduction rate and the egg reduction rate were 2 87%,29 62%,31 55% and 10 5%,45 00%,63 34% respectively Collectively a partialy immune protection against schistosoma japonicum infection was induced by intranasal vaccination with rSjGST-CA
出处
《中国热带医学》
CAS
2003年第6期713-716,共4页
China Tropical Medicine
基金
湖南省科委课题 (oojzy2 1 1 5)
