摘要
目的用重组日本血吸虫铁蛋白免疫小鼠，观察抗血吸虫的免疫保护作用。方法用重组的日本血吸虫铁蛋白（ＧＭ－ＣＳＦ－ＳｊＦｅｒｒｉｔｉｎ）免疫小鼠，经皮下免疫 ３次，在第 ３次免疫后 ４周，经腹部感染日本血吸虫尾蚴４０条或５０条，４５ｄ后杀鼠，观察减虫效果。结果用ＧＭ－ＣＳＦ－ＳｊＦｅｒｒｉｔｉｎ、ＩＬ－１２＋ ＧＭ－ＣＳＦ－ＳｊＦｅｒｒｉｔｉｎ和 ＦＣＡ＋ ＧＭ－ＣＳＦ－ＳｊＦｅｒｒｉｔｌｎ免疫小鼠分别获得 ２２． ８６％、 ２５． ６３％和３９ ８１％的减虫率。结论本研究首次证明铁蛋白能诱导抗血吸虫病保护性免疫。
Objective To test protective immunity against Schistosoma japonicum (S. j) infection induced by Sj Ferritin in mice. Methods Mice were divided into different groups and injected subcutaneously with GM-CSF--Sj Ferritin,IL-12+GM-CSF-Sj Ferritin,FCA +GM-CSF-Sj Ferritin. FCA or PBS, respectively and were challenged with 40 or 50 S. j cercariae. Forty-five days later. mice were killed and perfused,and the adult worms were counted. Results The reduction rates in worm burden were 22. 86 %. 25. 63 % and 39. 81 % respectively. Conclusion This study first reported that a significant protective immunity against Schistosoma japonicum infection was induced by vaccination with Sj Ferritin in mice.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2000年第1期12-14,共3页
Chinese Journal of Schistosomiasis Control
基金
本研究得到湖南省科委及TODR/WHO资助!(ID 80268)
关键词
日本血吸虫
铁蛋白
免疫
小鼠
Schistosoma japonicum, Ferritin, Immunization, Mouse
