摘要
目的探讨弓形虫可溶性抗原(STAg)和γ干扰素(IFN-γ)联合鼻内免疫对小鼠的保护作用及IFN-γ作为佐剂鼻内免疫抗弓形虫感染的最佳剂量.方法将5～6周龄BALB/c小鼠70只随机分为5组,每组14只,分别用20μg STAg、20μg STAg+250 U IFN-γ、20 μg STAg+500 UIFN-γ、20μg STAg+1 000 U IFN-γ和20μg STAg+2 000 U IFN-γ鼻内免疫小鼠2次,间隔14 d,末次免疫后第10天,用RH株弓形虫速殖子4×104个/只灌胃攻击,逐日观察小鼠健康存活情况.攻击后第29天处死全部小鼠,分离计数脑、肝组织速殖子;计数肠系膜淋巴结(MLN)和脾T淋巴细胞.结果随着佐剂IFN-γ剂量的增加,小鼠存活率有升高趋势,1 000 U IFN-γ剂量组小鼠存活率最高达93%;肝、脑速殖子数呈下降的趋势,其中STAg+1 000 U IFN-γ、STAg+2 000 UIFN-γ组显著低于STAg组;MLN和脾T淋巴细胞发生了增殖性应答,其中STAg+1 000 UIFN-γ、STAg+2 000 U IFN-γ组MLN细胞显著高于STAg组.结论STAg+1 000 U IFN-γ、STAg+2 000 U IFN-γ鼻内免疫明显优于其它小剂量佐剂及单独抗原免疫,能有效诱导黏膜免疫应答.
Objective To investigate the protective effect against Toxoplasma gondii infection following intranasally administered mice with STAg and gamma interferon （IFN-γ）, and determine the optimal dose of IFN-γ as adjuvant for intranasal immunization. Methods Seventy 5- to 6-week-old BALB/c mice were randomly divided into five groups, fourteen mice of each group were immunized intranasally two times at 14-day intervals with 20μg STAg alone, 20μg STAg plus 250 UIFN-γ, 20μg STAg plus 500 U IFN-γ, 20μg STAg plus 1 000 U IFN-γ or 20μg STAg plus 2 000U IFN-γ. The PBS containing 4×10^4 tachyzoites of RH strain in each 0.5 ml volume were orally administered to all animals on Day 10 after the last immunization. The healthy and survival of mice were observed day by day. Survivors were sacrificed on Day 29 after infection, the tachyzoites in livers and brains were counted, the number of MLN and spleen T lymphocytes were determined.Results The ＇survival rate of mice rised with the increasing dose of IFN-γ. The survival rate of STAg plus 1 000 U IFN-γ group was 93%, which was the highest among the groups. The tachyzoites had an incline to decrease, which were significantly smaller in the groups of STAg plus 1 000 U IFN-γ or STAg plus 2 000 U than those of STAg groups （P〈0.05）. Proliferative responses were observed in MLN and spleen T lymphocytes, and the MLN cells in the groups of STAg plus 1 000 U IFN-γ and STAg plus 2 000 U were obviously higher than those of STAg groups （P〈0.01）. Conclusion Intranasal immunization of STAg plus 1 000 U IFN-γ and STAg plus 2 000 U is prior to other groups, which induced mucosal immune response effectively.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2005年第4期293-297,共5页
Chinese Journal of Schistosomiasis Control
基金
山西省自然科学基金(No.20041105)
山西省高校科技研究开发项目(No.20041238)
山西医科大学基础医学院学科研究方向建设基金(20040708)
关键词
弓形虫可溶性抗原
γ
干扰素
弓形虫
鼻内免疫
黏膜免疫
Soluble Toxoplasrna antigen
Gamma interferon
Toxoplasma gondii
Intranasal immunization
Mucosal immunization
